Gene Transfer into Subcultured Endometrial Cells Using Lipofection
نویسندگان
چکیده
منابع مشابه
Gene transfer into subcultured endometrial cells using lipofection.
Lipofection using the Lipofectin reagent was optimized to transiently transfect subcultured guinea pig endometrial stromal cells with a beta-galactosidase gene driven by a simian virus 40 promoter. Efficient transfection was obtained in the following conditions: a value of six for the ratio of lipofectin to DNA, a low cellular density (10(5) cells per 35-mm well) at the time of subculture (48 h...
متن کاملGene Transfer into Sub- cultured Endometrial Cells Using Lipofection
Lipofection using the Lipofectin® reagent was optimized to transiently transfect subcultured guinea pig endometrial stromal cells with a β-galactosidase gene driven by a simian virus 40 promoter. Efficient transfection was obtained in the following conditions: a value of six for the ratio of lipofectin to DNA, a low cellular density (105 cells per 35-mm well) at the time of subculture (48 h bef...
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The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro development of somatic cell nuclear transfer (SCNT) embryos. Green fluorescent protein (GFP) gene was used as a foreign gene and a non-transfected somatic cell was utilized as a control karyoplast. Mon...
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Specific dihydrotestosterone (DHT) binding was demonstrated in the whole cell sonicates obtained from serially subcultured human endometrial fibroblasts. This binding showed a high affinity and specificity for DHT (Kd=3.3 X 10(-10) M). Various kinds of excess nonradioactive steroids were arranged, as a result of competition among them, in the following decreasing order of binding affinity: DHT,...
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The electric field method of gene transfer via electroporation of cell membranes is gaining importance in cellular and molecular biology. In particular the electroporation technique provides the low copy number transfection required fqr genetic studies. As demonstrated with mouse lyoma cells, with lymphoid cell lines and with plant protoplast, the electric method has appreciable advantages comp...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96201st03